Oct 18, 2016
from 02:00 PM to 03:00 PM
|Where||Genetics Part II Room|
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There will be an informal seminar held at 14:00 in the Part II Room on Tuesday 18th October 2016. Professor Frank Albert from the University of Minnesota will be speaking and the title of the talk is, ''Towards a complete view of the genetics of gene expression variation in yeast''
Genetic variation among individuals is an important source of phenotypic variation within species. Many of these genetic effects are due to regulatory variation that influences gene expression. However, the full extent and nature of regulatory variation remains unknown due to the limited sample sizes that are typically studied, resulting in low statistical power.
I will discuss our ongoing work addressing this limitation for the proteome and the transcriptome in the yeast Saccharomyces cerevisiae. For protein levels, we devised an approach based on extremely large pools of genetically different cells that carry proteins with fluorescent tags. For mRNA levels, we used RNA sequencing to profile gene expression in more than 1,000 recombinant yeast individuals. The statistical power of this dataset is high enough to map thousands of regulatory loci that together account for ~80% of the heritability of mRNA levels. Together, these data provide a nearly exhaustive view of how genetic variation influences gene expression.
Host: Chris Illingworth